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mab6308  (R&D Systems)


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    Structured Review

    R&D Systems mab6308
    Mab6308, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mab6308/Human+Collagen+IV+alpha+1+Antibody/pm41398913-149-23-24
    Average 93 stars, based on 4 article reviews
    mab6308 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Neurotrauma induced retinal basement membrane COL4A1 defects are restored by adipose tissue derived mesenchymal stem cell concentrated conditioned medium.
    Article Snippet: The membrane was blocked with 5% bovine serum albumin in TBST for an hour, followed by incubation with primary antibodies (COL4A1, Cat # MAB6308, R&D Systems; TSG-6, Cat # AF2104, R&D Systems; TIMP-1, Cat # ab211926, Abcam) and subsequent detection using HRP-conjugated secondary antibodies.

    Article Title: Neurotrauma induced retinal basement membrane COL4A1 defects are restored by adipose tissue derived mesenchymal stem cell concentrated conditioned medium
    Article Snippet: The membrane was blocked with 5% bovine serum albumin in TBST for an hour, followed by incubation with primary antibodies (COL4A1, Cat # MAB6308, R&D Systems; TSG-6, Cat # AF2104, R&D Systems; TIMP-1, Cat # ab211926, Abcam) and subsequent detection using HRP-conjugated secondary antibodies.

    Construct:

    Article Title: 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization
    Article Snippet: Intracellular targets were stained according to the protocol described by Dekkers et al. [ ] In brief, constructs were fixed in 4% PFA for 45 min at 4 °C, rinsed for 10 min in PBS with 0.1% Tween20 and then blocked for at least 15 min in wash buffer, consisting of PBS with 0.1% bovine serum albumin (BSA, Miltenyi Biotec, 130‐091‐376), 1% Triton X‐100 (v/v), and 0.02% sodium dodecyl sulfate (SDS, Fisher BioReagents, BP1311‐1) at 4 °C. .. Constructs were incubated overnight at 4 °C on a rocking plate in primary antibodies, diluted in wash buffer as follows: TUBB3, 1:500 (Abcam, ab18207), Vimentin, 1:500 (Abcam, ab20346), COL4A1, 1:100 (R&D Systems, mab6308), LAMA1, 1:100 (Invitrogen, pa1‐16730). .. The next day they were rinsed with wash buffer for 2 h each at 4 °C on a rocking plate, then incubated overnight with secondary antibodies, DAPI and pre‐conjugated phalloidin, diluted as follows: Goat anti‐Rabbit Secondary Antibody Alexa Fluor Plus 488 (1:200), Goat anti‐Mouse Secondary Antibody Alexa Fluor Plus 594 (1:200), Goat anti‐Rabbit Secondary Antibody Alexa Fluor Plus 647 (1:200), AlexaFluor Plus 647 Phalloidin (1:200, Thermofisher, A30107), and DAPI (1:1000).

    Article Title: 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense in PBS with 0.1% Tween20 and then blocked for at least 15 min in wash buffer, consisting of PBS with 0.1% bovine serum albumin (BSA, Miltenyi Biotec, 130-091-376), 1% Triton X-100 (v/v), and 0.02% sodium dodecyl sulfate (SDS, Fisher BioReagents, BP1311-1) at 4 °C. .. Constructs were incubated overnight at 4 °C on a rocking plate in primary antibodies, diluted in wash buffer as follows: TUBB3, 1:500 (Abcam, ab18207), Vimentin, 1:500 (Abcam, ab20346), COL4A1, 1:100 (R&D Systems, mab6308), LAMA1, 1:100 (Invitrogen, pa1-16730). .. The next day they were rinsed with wash buffer for 2 h each at 4 °C on a rocking plate, then incubated overnight with secondary antibodies, DAPI and pre-conjugated phalloidin, diluted as follows: Goat anti-Rabbit Secondary Antibody Alexa Fluor Plus 488 (1:200), Goat antiMouse Secondary Antibody Alexa Fluor Plus 594 (1:200), Goat anti-Rabbit Secondary Antibody Alexa Fluor Plus 647 (1:200), AlexaFluor Plus 647 Phalloidin (1:200, Thermofisher, A30107), and DAPI (1:1000).

    Incubation:

    Article Title: 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization
    Article Snippet: Intracellular targets were stained according to the protocol described by Dekkers et al. [ ] In brief, constructs were fixed in 4% PFA for 45 min at 4 °C, rinsed for 10 min in PBS with 0.1% Tween20 and then blocked for at least 15 min in wash buffer, consisting of PBS with 0.1% bovine serum albumin (BSA, Miltenyi Biotec, 130‐091‐376), 1% Triton X‐100 (v/v), and 0.02% sodium dodecyl sulfate (SDS, Fisher BioReagents, BP1311‐1) at 4 °C. .. Constructs were incubated overnight at 4 °C on a rocking plate in primary antibodies, diluted in wash buffer as follows: TUBB3, 1:500 (Abcam, ab18207), Vimentin, 1:500 (Abcam, ab20346), COL4A1, 1:100 (R&D Systems, mab6308), LAMA1, 1:100 (Invitrogen, pa1‐16730). .. The next day they were rinsed with wash buffer for 2 h each at 4 °C on a rocking plate, then incubated overnight with secondary antibodies, DAPI and pre‐conjugated phalloidin, diluted as follows: Goat anti‐Rabbit Secondary Antibody Alexa Fluor Plus 488 (1:200), Goat anti‐Mouse Secondary Antibody Alexa Fluor Plus 594 (1:200), Goat anti‐Rabbit Secondary Antibody Alexa Fluor Plus 647 (1:200), AlexaFluor Plus 647 Phalloidin (1:200, Thermofisher, A30107), and DAPI (1:1000).

    Article Title: 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense in PBS with 0.1% Tween20 and then blocked for at least 15 min in wash buffer, consisting of PBS with 0.1% bovine serum albumin (BSA, Miltenyi Biotec, 130-091-376), 1% Triton X-100 (v/v), and 0.02% sodium dodecyl sulfate (SDS, Fisher BioReagents, BP1311-1) at 4 °C. .. Constructs were incubated overnight at 4 °C on a rocking plate in primary antibodies, diluted in wash buffer as follows: TUBB3, 1:500 (Abcam, ab18207), Vimentin, 1:500 (Abcam, ab20346), COL4A1, 1:100 (R&D Systems, mab6308), LAMA1, 1:100 (Invitrogen, pa1-16730). .. The next day they were rinsed with wash buffer for 2 h each at 4 °C on a rocking plate, then incubated overnight with secondary antibodies, DAPI and pre-conjugated phalloidin, diluted as follows: Goat anti-Rabbit Secondary Antibody Alexa Fluor Plus 488 (1:200), Goat antiMouse Secondary Antibody Alexa Fluor Plus 594 (1:200), Goat anti-Rabbit Secondary Antibody Alexa Fluor Plus 647 (1:200), AlexaFluor Plus 647 Phalloidin (1:200, Thermofisher, A30107), and DAPI (1:1000).



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